What Does Hallatosis Smell Like

When the art technology Michaelis-Menten killed

start with the end, let us dead something that falls into disuse. Well this man, who in reality were two gentlemen not only are dead for many years, but here I am talking about his legacy. For those initiated into the life sciences, the Michaelis-Menten enzyme kinetics are not unknown, and most of clairvoyance to remember and why they wore, what we remember is that they were a nightmare, both to understand in the classroom like to make in the laboratory. For the uninitiated, is basically a way to study the characteristics of enzymes , which are nothing more than proteins that can facilitate a chemical reaction.

This type of kinetics was developed in the early twentieth century by Dr. Leonor Michaelis and Dr. Maud Menten , and is, at least for me, the paradigm of testing in vitro . I will not go in their complexity and mathematical assumptions. Their experimental procedure and roughly summarized: put an enzyme into a pot along with everything needed to make it work and at one point was added the substrate that the enzyme has to modify (phosphorylate, cut, break, destroy, ubiquitin etc etc. ..) and start timing. Note, that prior to this we have: purify a stable enzyme functional and invented a method to measure substrate disappearance or product appearance and standardize on a host of conditions that may affect the enzyme (ionic strength, pH, temperature, substrate concentration ...). This approach (as amended) has been used for almost 100 years to measure the kinetic constants (characteristics) of all known enzymes and purify them likely to be active in a boat. And it has been of tremendous use from the science of knowing in learning to pharmacodynamics (which saves lives). But the laser was the "in vitro " is coming to an end (let me use part because by all in the title).

Indeed in vitro experiments are still far from disappearing, but new technologies, most of those who are dedicated to studying the mechanisms of life, we are changing the boat in the living cell. Advances in genetic engineering, along with the discovery and development of new biological tools such as proteins and fluorescent substrates and very powerful microscopes (in addition to its highly refined optics with computerized control systems that allow the manipulation of micron level) do may be almost easier to follow the road map intracellular your "beloved protein X" to make a simple mix in a jar. You lose total control of all parameters of the reaction (in vitro fails ) but you gain, among other things, perceptions of what happens in "real life" also allows the analysis and quantification of processes as intracellular protein movement, or simple protein interactions in native environments (ie natural), that in vitro are almost impossible to discern.

green fluorescent protein (GFP image above), it absorbs blue-violet light and emits green light. Was obtained in the first version of the jellyfish Aequoria victory (abaj0). Successive improvements in genetic engineering have generated more stable types of GFP and bright, they are more useful.

This technology opens the closure of the study on single cell protein, is going to take a boat ( in vitro) data which reflect the overall statistical behavior of a population of millions of molecules, proteins or thousands of cells, to study tens or hundreds of molecules in a dozen or twenty of cells, but in depth. Here we realize that all behave in similar but not identical, which gives much more information. At the restaurant as an example the New York marathon. Speed \u200b\u200bstudy the long distance race of human beings. In an in vitro classic, taking into account all the people, collectively viewed from afar, who ran the last marathon (45 334), conclude that the speed of human being, 40km, is around 10km / h. But using this new cutting edge technology we are able to adjust much more and we can study the runners one by one, 20 of them for example to see that some are at 19 km / h, more than 10 km / h and other 7km / h. But let implausible examples and see some real examples:

fluorescence microscopy: That will be the base. In short, what is done is to attach a particular protein (X) to an existing fluorescent proteins such as GFP (green emitting) or Cherry (emitting in red). X (-GFP, for example) is expressed in the cell, the cell is put under the microscope, and hopefully that will be part of the cell is located our protein X (now green or red). And even if the protein moves, you can follow their movement over time (fluorescence microscopy in living cell).

Scheme how it makes a protein X either a fluorescent mark in this case GFP ( image )

real example fluorescence microscopy. Different cell types (rat skin, carcinoma ...), where the same protein is marked with GFP ( image )

FLIP: This acronym abbreviates the definition in English (I will not use it Images are available). This technique is called something like "fluorescence loss in photobleaching. Here is what is done as in the fluorescence microscopy but also living cell, using a laser is extremely precise "char" (photobleaching) continuously fluorescent protein together with our protein X in a small part of the structure are fluorescent staining. If the progress of time, the fluorescent signal is lost outside the area of \u200b\u200bphotobleaching, this means that our protein X blithely moves from one side to another, and when placed under the sun ... I mean, Laser tarnish. In short, moving the structure to make, and is not static.

FLIP and FRAP diagram, the rectangle indicates the area Cell to photobleaching (in English, photobleach) in each case ( Jennifer Lippincott-Schwartz and George H. Patterson, 2003, "Development and Use of Fluorescent Protein Markers in Living Cells. "Science )

real example of FLIP (now the image is in black and white because you have to pay to publish less, and in this case information is the same.) The square indicates the area to be "char" (photobleaching) continuously. In the second picture starts photobleaching Sool in the rectangular area, you can see how the fluorescence gradually disappears from the rest of the cell. (Jennifer Lippincott-Schwartz, Erik Snapp and Anne Kenworthy, 2001. Studying protein dynamics in living cells. Nature)

FRAP: "fluorescence recovery after photobleaching. It is like FLIP it in reverse. "Burn" the fluorescent protein in an area and waiting to see how (yes or no) fluorescence recovers.

real example of FRAP. The square indicates the area to be "char" (photobleaching) but in this case only for a short period of time. The three photos above are for a mobile protein, for 5 min. after the "charred", the area in question again becomes fluorescent. The three images below are a control, doing the same procedure in a protein that is not mobile, we can see after photobleaching the fluorescence remains square area. (Jennifer Lippincott-Schwartz, Erik Snapp and Anne Kenworthy, 2001. Studying protein dynamics in living cells. Nature)

FRET: "induced fluorescence energy transfer." The theoretical implications of this technique are more complex physically, but the idea is simple. A molecule is fluorescent light because it contains only one type (eg blue, in the case of GFP) and then the other issues but with less power (in this case green). Well, what happens if we use GFP with another protein that emits green light and reflects red (eg CHERRY), for if they are sufficiently close to each other, GFP can move to green energy and this issue CHERRY red. So if we want to know whether two proteins (X and Y) interact in their natural environment, joined to a GFP X and Y to Cherry and get into a cell. As in fluorescence microscopy, we will be able to see the location of each one separately if you use the "activating light" concrete of each. But if we use the green light to activate GFP and what we see is red (or yellow = red + green) shows that out of our protein indicates that our protein X and Y are very close (as Batablanca and Malina at some point) ... interacting (and not be wrong-headed).

Example FRET. This is another approach of this technique can observe how the shape of a protein under certain conditions (such as shortening the length). A, control. B, a protein fragment is bent causing the fluorescent protein approach. C, if the fragment is shorter, there is more efficiency in energy transfer between GFP and Cherry ( David Lleras, Samuel Swift, Angus I. Lamond, 2007. Detecting Protein-Protein Interactions In Vivo with using FRET Multiphoton Fluorescence Lifetime Imaging Microscopy (FLIM). Current protocols )

is true that after reading this tirade about these super-technological techniques, which are very impressive, one concludes that appear not serve nothing but to entertain a heap of scientists fascinated by the bright colors. However, their application, along with a host of other techniques (more or less leading and finicky), is enabling a deep understanding of the mechanisms of life. And this is important for many things, but two are key from my point of view. The first is that the mere knowledge (if only to know how is a bacteria or yeast ) is not a waste of time, although today not yield economic benefits or not know what it does or will. These techniques (and other, non-microscopic) is rooted in scientific and technical developments initiated 20 or 30 years ago (when they were only to be done), today are the tip of the spear which aims to eradicate Alzheimer's, cancer, AIDS, hepatitis, diabetes, malaria and a long list of pests that plague our world today. And the second is that all this search-application of knowledge is, at least from publicly funded research (at least in the 10 laboratories that have stepped in my life), applying a level of caution and environmental and personal protection that if you take into other jobs or everyday life, the most we had not cut with a knife in our lives.

Can I Make A Copy Of Px90

The Precautionary Principle

few hours ago, while reading the newspaper (unhealthy and depressing activity where there is) there is an article that has been an increase in synaptic activity of my neurons, and it was not news: the development of genetically modified cows to produce milk more humanlike, which is more suitable for infants both nutritionally and because they provide some protection against some types of pathogens (more info here), the fact that caught my attention was the argument of the opposition groups against this particular application and against GMOs in general: the precautionary principle .

Exemplification of principle if burned fear that we would not have used the Windsor fire, we would not be where we are (image and concept by Professor of Biochemistry and Molecular Biology of Plants, UPV, Oscar Vicente)

This argument, which in a nutshell is to "do anything you're not 100% sure that is not going to be dangerous, just in case", it is becoming more common in protests against this kind scientific advances. This argument at first, so logical and sensible can be a double-edged sword, as it leads to stagnation, fear and complacency. Consider for example the first aircraft and submarines, it is clear that no one went in the first metal frame built, but I doubt that at the time of the first human trial had full assurance that the device in question is not crash or bankrupt (as appropriate) in the middle of the journey, the same could be said of the first shot, I do not Edward Jenner had the complete confidence that the smallpox inoculation to the first patient with whom the vaccine was tested this is not to the disease, the same way I can get by going back to the past, such as the fact that despite the large number of traffic accidents that occur every week (I will not get to give figures, here the statistics and extrapolations is the friend Banchsinger ) no one has asked yet outright ban of cars and, moreover, we can trace to our very origins, when someone discovered the fire, if that prehistoric nice not "would have played" and played with fire (hence the expression comes in handy), you might now we were not where we are.

So forgive me if I ask politely that strictly in favor of applying the precautionary principle again to the caves and leave us scientists to deal with our affairs. This is not to say that the good things done haphazardly just because we can, but science has never been so advanced as now, and with it the risk assessment methods and environmental impact, so I apologize if I disagree on that if a technology that, due to the high social pressure against it, has overcome many more checks and controls as any other system or improving food production, if it is not a problem nor is the coast clear, I doubt that suddenly becomes harmful Satanic or apocalyptic, so forget biblical fears and stop-ahead to progress. The precautionary principle is applied as a dogma of faith goes against the very essence of man, restless and active, always pushing us to improve, to make further progress, nay, and I apologize for who may be offended, but that kind of reminds me of immobility some past situation, in which a controlling church was opposed to any scientific breakthrough that would contradict with previously accepted. Genetic improvement has traditionally becoming centuries (since the beginning of agriculture and livestock), the use of genetic engineering, just the next step of an ancient road, a fine and accurate tool to achieve the same objectives and other new, with whom we never even dared to dream. Science is not perfect, probably never will be, but I sincerely believe that is the best tool we have to keep evolving. Applications to be given to this technology may well be questionable; the technology itself, no.

Can I Give My Dog A Stool Softener

Batablanca - Chapter 9: Jindetrés out

BATABLANCA ABOVE: Meeting with Bam in the tavern de Sac, Batablanca tells how his old missions intracellular Exbauno, lost his temper when he fell in love Batablanca Malina. The seductive protein had interfered between the two partners, and the unstable triangle ended with a fight between Batablanca and Exbauno, which was mistakenly left for dead. In fact, Malina was forced to hide that Exbauno still alive (under the new identity of "Jindetrés) for fear of reprisals from it. The revelations Batablanca end with saying that only can be found if you rely Jindetrés Malina, who promised to bring him (Chapter 6: Cups, revelations, and a challenge) . Our heroes are pipetted in a transfection, and get into the cell with the help of JAK, a longtime collaborator of Batablanca. However, entry does not go unnoticed and a sudden activation of the receptor, with subsequent cascade of phosphorylation, is about to finish them ( Chapter 7: Finding JAK). Once in the cell infiltrates, move along the microtubule network but are detected by antibodies that are about to end Malina, rescued by Bam in the last minute. After pausing to reflect, Jindetrés conclude that our heroes must be found in the nucleus, where he has been monitoring what was happening inside the cell. So, are directed toward it, but started from the endoplasmic vesicle covers Bam and it's in retrograde transport. Although Batablanca comes to his aid, unable to reach the gallbladder, which is lost in the distance. Batablanca, angry, back to the outskirts of the challenges and Jindetrés to come out of hiding ( Chapter 8: Strangers in a microtubule ).

For more than repeat my challenge, did not get response. The comings and goings of vesicles was pending, and the complexes are assembled and disassembled around me, seemingly indifferent to what was happening. On more than one occasion I thought that one of the vesicles open suddenly, to show Jindetrés inside, but never happened. He was about to give up and give everything to lose, when I saw it.

High in the nucleus, next to a nuclear pore nucleoporins where seething still Jindetrés looked at me in silence. From that distance I could not say for sure, but I had the impression that nothing was altered. Absolutely. That bastard ... always admired his moderation, his nerves of steel in critical situations. When we worked together, it was a great advantage, now ... Well, now simply was scared.

I stood as much as I could. I could not let myself be intimidated, he noticed how scared I was. Could try to cheat and say I was angry rather than frightened, angry, anxious for revenge, but no, I was really scared. I could not take my mind the image of being engulfed by Bam gallbladder, and all of a sudden other images succeeded: JAK autophosphorylated the body Pistuno semiproteolizado of even the remnants of degraded plasmids formed the first clues. All this violence, all these deaths and changes in processes normal birth were caused by the same protein that rose, stunning, over the cell nucleus which had full and free access. Yes, I will not deny: he was terrified.

But he could not say that was new in this business. It was not the first time I was terrified the nerves gripped my every amino acid. I knew I had to do. Standing on my entire molecular mass, adjusted my coat and my hat, I reorganized my alpha helices and became all the fear and tension in potential energy to go ahead and use it to my advantage. I jumped in ribosome ribosome, never at any time in the light of Jindetrés. I could see how he clenched his fists and did the same, jumping in my direction. Inexplicably, I could not help but smile: in one way or another, all would end soon. It was time for the truth.

The shock was brutal. Jindetrés I almost doubled in size, but I had taken enough momentum for the onslaught was no laughing matter. Our departure terminal end is tangled, preventing us to separate. Struggling, fell and bounced from the nucleus to the lattice, colliding with all the proteins that came and went. Finally, our erratic career was abruptly stopped, the blow was so strong disentangle us and we fell apart a few microns of each other. Stunned, we look around and try to understand what had fallen. It was a kind of platform, which was moving slowly. He seemed composed of proteins, but also could glimpse fragments of ribonucleic acid. I had a shock to think that it was a virus particle, just as he lifted my left I saw a similar structure, not far away: so breathe easy. "Proteins vault ..." - I said - "... I have no idea what the hell to serve, but the truth is that as airstrips have no price. " The stability of such macrocomplex allowed me to get up and relocate my battered domains. Fortunately none of my amino acid was hydrolyzed, and although it hurt even more hidden glycine was over. Unfortunately, I learned that Jindetrés was equally large. And how he rose fast, certainly less painful than a server. To confirm my suspicions began to laugh loudly.

- Ah, Batablanca, you were always a stubborn and a bully. Unable to recognize when you take everything to lose. And stubborn as you alone. I am sure that even Have you considered my gallant offer to join my cause. You have chosen to throw his fists rather than dialogue. Who is the villain, then?

could not believe it. The very manipulative. If you wanted to forget all the times he had tried to demote me, you do not really know me. Probably just wanted to get me mad. Or maybe ... maybe to feel some remorse way. Do you really expect me to join him, to not have to kill me? Had some old Exbauno still latent protein that terrible and violent?

- Join your cause? What is not that, friend? - I tried to give my words of a turn aggressive, unpleasant. - Do you want to become master of the cell? Manipulating each and every one of its components as you please, for your benefit? "Digesting any plasmid that does not abide by your designs and proteolyzed to those who do not follow suit? Is that what you call a "cause"? You must be crazier than I imagined. I do not know how you got rid of almost certain death, but it is clear that what was there escaped Exbauno, and not just because you changed your name. Exbauno knew what it meant to be someone's companion. He did know a mission and had never even thought of killing for it.

Hearing the old name had the effect I expected. For a moment I believe - made him hesitate, but then seemed even more angry. He ruffled the lysines in the central domain. He shouted.

- Forget that name! Yes, you might Exbauno died, killed at least everything that did not serve him. Now I'm better, bigger, louder, I can do things you never imagined. Look around, Batablanca: what do you see? Chaos! This whole network of molecules that surround us What are they? Whom do they serve? No one, did not you see? Operate automatically, without meaning, without a mission. Someone has to control all this. My old self would never have been able to dominate the cell and its mysteries ... but since I was reborn, there is nothing that I resist. Before I could do to cut only DNA ... and almost do not remember, what unhappy fate! My breakdown would have been a relief, in retrospect. But now I'm capable of much more, that's why I could not just live in the eppendorf ... there is no future there, Batablanca, but here in the cell can do whatever we want. We control a powerful machine, and only this way will know our true destiny ...

definitely was completely mad. But while listening to his delusions, I realized something amazing ... Jindetrés went on and on talking non-stop, explaining their motivations and their absurd plans, leaving me time to recover and think of a way out of that mess. Before me was the opportunity I was needing desperately. While the very mouthy continue behaving like a comic-opera villain, may have a loophole. So I took the opportunity:

- But what are you saying? Do not even know how you could be "reborn" in place of demoted ... do you really think it was something random? What you alone are master of your destiny? Do not make me laugh! What we are is a bully, your destiny is as strong as other proteins that surround us, that of the ribosomes that are translating around us without flinching, that of the dynein and kinesins that move incessantly, even in the vaults on which we are now ... whatever you do.

My trick was effective; Jindetrés left his state of tension to launch a laugh and continue expounding:

- What poor fool has always been, Batablanca! To think that I even admire you ... now I realize how terrible and cowardly it is your nature: you've ever wondered about who you really are. You've just followed orders, to fulfill your job. But you never tried to reach your full potential. So do not deserve, and I regret to say, join me in my mission. While

went on and on talking non-stop, scoring the surface of the vault on which we had fallen. My hopes were rewarded: I stumbled across a terminal end of a chain of MVP who had separated from the rest: it was a pair of electrons to discard the next monomer. A slight pressure, and separated from everything. Slowly, subtly beneath the folds of my gown, I was tearing around the polypeptide, as if it were a zipper, while biting the loudmouth was my enemy.

- It's amazing how you speak in your mission, if you could see how ridiculous that sounds! I think in reality you are scared, and in your arrogance you think you can get away from your sad fate that has been and always will be ... be a simple and innocuous restriction enzyme ...

This time gauge my bad words, and endurance of Jindetrés. What a rookie mistake, I thought then directly attack his ego in such a tense situation. His face went from sarcasm to dead serious. He clenched his fists and bellowed

- Damn idiot! I'll decide what my fate! I, and nobody else!

not be writing these lines - that's for sure - if the reflexes were not one of my greatest virtues. While all of Jindetrés kilodaltons made against me as a tank, pulled by the roots MVP rod and struck him brutal that tore several side chains, which made him stumble off balance and to disappear from the top of the vault .

It was so fast that I was petrified, not daring to join. He kept pushing the piece of MVP so strong that it hurt. For a moment I could almost imagine it was all over, but something inside me told me it could not be easier. I finally got up and walked carefully to the end of the platform. I peered cautiously over the edge and I could see down there on a folding of the reticle. Was whole, but bruised. And angry. Very angry. A cry I surprised my back before deciding what to do next:

- Batablanca! Here!

Malina. I had forgotten all about her, and there was, on another vaults floating in the cytosol. Doubtless he had been trying to get to us, and had witnessed the fight.

I threw away my makeshift weapon, and jumped in his direction. I noticed that they kept us floating around more and more vaults and colorful complex macromolecular vesicles of all kinds ... something moving in the cell. I did not like. With a last jump I stood next to Malina, launched into my arms. Hug him back firmly, but still see around me. She felt the tension.

- What has happened, Batablanca? Jindetrés "... is ...?

- is alive. And not very happy, dare I say - I said, without giving him time to finish his question. He gave me the impression that she was relieved, but at that time could not say for sure.

- Malina - told - whatever that aims Jindetrés, will not be good for us. We must get out of here as soon as possible. I think things will get ugly quickly.

This time they showed signs of nervousness when I answered:

- But I thought we were going to stop Jindetrés ... Batablanca, if we will pursue us, again could not bear the tension, uncertainty, knowing that you are watching but I can not see! You have to kill it, I thought ... I thought I could trust you!

I stared at her. He looked so vulnerable ... suddenly not so worried about what I was going to happen: just wanted to make her feel safe. She returned la mirada. Durante unos microsegundos nada más existió, y nuestros rostros se acercaron. Me abrazaba más intensamente que nunca.



Entonces, sentí un fuerte impacto a mi espalda. Todas mis cadenas laterales se estremecieron, y me separé involuntariamente del abrazo de Malina. Ella me miró, pero su rostro no había cambiado en absoluto. Seguía observándome con expresión dulce, frágil y preocupada. Poco a poco se alejaba de mí… no, en realidad yo me alejaba de ella.



- Lo siento, Batablanca – dijo, en un susurro. – De verdad te amo, pero… no puedo luchar contra lo que soy.



Arrastrado por la cadena de poliubicuitina que was now part of me, I could see a ubiquitin-conjugase Malina that aside, once I had downloaded on his cargo. Malina stood there, looking in my direction. When I finally got out of my stupor, I tried to rip the damn all costs ubiquitin, but my efforts were in vain. I could not reach them. I squirmed stupidly, without getting anything else to spend the little energy I had left. I looked around, desperately trying to find some desubicuitinasa among dozens of proteins that are constantly moving around me. It was then I realized that it was not worth fighting. In front of me, an army of caspases roamed the cytosol, degrade substrates in their path: the heart vibrated in its entirety, and the few proteins that could have any chance be heading rapidly to the plasma membrane. "All right - I said - I can choose between dying degraded in the proteasome or perish with the cell and all its contents. Nor is it a difficult decision. " It may seem the result of pride, but really little I care and my destination. All I could think Malina. In your face, your last embrace ... his betrayal.

A shadow me out of my thoughts. I glanced behind me to see that soon all would end, my back, the proteasome stunning rose, prepared to fulfill its mission.

In this case, make Batablanca, Research Cell, small bits of amino acids.

conclude !

A Letter To Disconnect

Today is my birthday, or where are the Freaks?

Yes, today is my birthday, but not one that the future of brand on fire in my stem cells. This week like this a year ago I came to this land of Teutonic. I told here more or less absurd incidents and / or comic of the early days and months, but after a year in Germany, and although I am not given to making statements, today I'm going to make the experience here, trying to leave out of anger episodes our future situation and using accumulated anecdotes and incidents in recent months. Note, however, to take stock for someone with mental imbalances transient (how could it be otherwise in this blog) is not easy.

Language, is the first thing you and through you contact him. Anyone who has spent some time in his life in a foreign country whose language they know, know what it feels like an asshole. Here, and in my case twice, first, that although in English (if the mine can call it) is run, around here you realize that anyone speaks English street light years ahead of you since ( passer to your boss, which in Spain does not happen in the vast majority of cases). And then, because every time you want to order a loaf in his own Germanic language (which for a course that takes 6 months), and you learn the phrase, intonation, and the twelve possible answers, you go to the bakery, ask the bread and bread rather than receive, or an automatic change to the English shopkeeper, or at least sometimes, a question of the shopkeeper / her assistant youngster asking if he speaks English well, that I attend (and speak bakery because the times I've had this happen, the experiment under the same conditions, is a statistic that exceeds the most demanding test). This means that the only way forward is feeling asshole over and over again until you lose the shame of it all, and are able to talk to your boss from an Excel file with data from the last experiment, where you refer to an excel sheet as "shit" (shit) instead of "sheet" (sheet) (okay, the data were shit, but they are forms, dammit ...). However, since the first contact, you realize that, despite everything, people with whom I have dealt with are polite, friendly and helpful, and most will understand or seek to understand you the wrong way to explain yourself. Either way, these absurd thoughts aloud, who both gave the game to liven up the boring old days of mini or maxipreps seasoned with a gel, are lost. And those that do very yours saying attributed to many, but that seems to come from Arab wisdom, which says: "If you ever open your mouth, your words are worth more than silence."

After things how are you come to the conclusion of one: what they would teach English Costao conditions (like here do), and two, that if you go postdoc morally unscathed, albeit with no result in "paper", personally, can give you satisfied.

not go much in the stock weather, which although important in the mood for it and the pages devoted to this purpose. Suffice it to say that even I, who am of Teruel, I run to get the horns in the sun when it peeks even timidly. Moving on scientific and "laboratoril", the first thing that struck me (apart from the economic capital invested in research, which is brutal) is that there are mixed techniques and more expensive machines with less expensive procedures, manuals and rudimentary, always seeking balance between production, cost and environmental pollution. As an example, if in a laboratory (one, of the University, with 20 people at most) is to buy two fluorescence microscopes (between the two, more than 300,000 Euros) as purchased, together with HPLC and other serenade expensive and useful gadgets. But yes, pipettes bacterial and yeast cultures, glass and autoclavable, and minipreps made as before, without plastic columns, and phenols, and nothing more polluting than EDTA (protocol of 14 steps and more than 1 hour and 30 min. for 12 minipreps). I have not stopped to review the budgets of fungible (material disposable), but the modus operandi is also reflected in the behavior and social functioning administrative and general. This leaves the impression that by being "forraos" paste to the ceiling, do not waste if it can be avoided. This denotes a personal and institutional responsibility that I have not seen too much out previously.

Another surprisingly large capital invested in science is human capital. Figures do not know beyond that in recent weeks have appeared in the English press. They spoke of the number of highly qualified personnel needed Germany to meet their needs and demand abroad. At the restaurant's playful comparative example the following situation. Years, not many, I was happily secure my brand new bike to any insurer that abound in our cities. When the profession I wonder where I worked (pre-doctoral researcher), after my response, my partner looked at me in amazement and wonder (like seeing a Martian Cassiopeia), after seconds of silence he said: "That's what ? "and I said scientific research, to which he replied:" I have many years making policies, and I swear that I never crossed with that profession. " Well then, when after 5 months here, I crossed half of Europe in I no longer new, nor brand bike, and I'm exposed to the same situation (this time at an insurance company with an unpronounceable name), give to the same question the same answer, and instead of receiving looks (that's is a freak) receive an automatic discount on the policy to be part of the scientific university. And I say, if you have a discount for that ... "Joer" is that here is like me in abundance. And indeed, not a week that I turn in the city with someone I know, even light, my body of research at the university.

Another thing quite bizarre to close my eyes accustomed to working in a laboratory with a density similar personal the mass density of a white dwarf was found laboratories where to go to work, you must first do a marathon under two hours. They are so big that I have legs like those of Indurain from walking every time I forget to take the pipette to load a gel (I'm going to patent the waist belt, biochemistry laboratory version, such as construction workers but adapted for research personnel used a small laboratoires). After spending years experimenting and carrying the westerns as if we were playing "twister" or houses of cards, this Labora-stage at first seemed to release steric, in fact no longer so cool. Personal interaction (for better and for worse) falls exponentially with distance. Silence, no comment or laughing or crying, people go, but the lack of a language common fluid, and less likely "interacionil" of the Nordic and Asian ends up making a dent in someone who has spent 5 years without knowing if the arm he came out of the armpit was his or one of his colleagues. The good part is that now there is nobody to hear me swear in Hebrew Teruel that shakes the pillars of the earth, and if by chance someone comes along ... for it is precisely that, Hebrew, so there is no terror in their faces .

know already, is vox populi , the German grid procedure, which has as one of its results, very commendable indeed, its superb engineering. Everything is planned, nothing is left to the dedicated improvisation in which Latinos are so adept. At first it seemed that they were so squared. But one day when, despite having a workbench as big as a tanker, filled it with potions, gadgets and potions, I discovered (by the pressing need for space for one of these experiments interspersed always come at an inconvenient time) that under my bed, huge wardrobe cabinet, had wheels, ruedassss!. So I shed the shank, and magically appeared 3 / 4 parts of bed for experiments in bulk. Throughout the day, everyone who passed, from the newest to the oldest, broke his silence with hallucination exclaiming: "Was ist Das?! Guaaaa an extra place to work ... great !!!". Yes, nobody had thought before, not lack of intelligence, that's for sure, but for lack of need (they do not overlap are experiments, and not because they do not), and moreover, the drawer was designed to be under the bed, not as a stadium-sized round table. What would say if they saw how sujetábamos maxiprep columns, there where I come from?

But what fascinated me most was basically between these observations has been ... the virtual absence of Geeks!, yes ... no geeks ... Where are the lab geeks so widespread in the few small English laboratories. Those partners with strange hobbies that are not widespread among the majority of the population. Those who write blogs more or less serious, silly. Those people who talk among experiments, or in coffee (I said I did not talk much, but among them whether spoken, or broadcast strange noises) of unlikely subjects. At first I thought yes I'd freak, what happened was that he did not know what they said then, not differentiated. But when after a few months German language course, pay attention, only hear conversations, if not experimental tasks revolve around, revolve around football, holiday weekend, beer, or purchases, and sometimes women, or timeliness ( elections, nuclear, soccer, weather ...). Where are those posters, banners, drawings and short written and hilarious that I have often seen in laboratories that have trodden before. Where is the wallpaper of your colleague was doing Kung-Fu, with the yin-yang ball as a logo or banner. Where are these gowns tuneadas as if it were a Harley. Where are those colleagues who visit websites of foreign content is not pornographic. Here I have not seen any of my colleagues outside of strictly scientific pages ... tion, yes, my colleague Schneider (bright 26-year doctoral student who sits at my side, separated by five feet of table), which as I've seen him 2 times a week, is to visit their facebook for 3 minutes at the most, or a motorcycle spares page another 5 min. We are 20, and only one, forcing the situation that would class as a geek (and I do not get as geek or the sole, and that I'm only amateur compared with other ... ahem, ahem), his sin is that it has a legion of carnivorous plants at home and plays the trombone. If geeks are hidden, not displayed in the workplace, which leads me to reappoint the seriousness of these people, I say no more professional or more workers (the volume of work per person, not time used, is practically the same thing I've seen before in other laboratories), I just think that separate the different aspects of life in a way that freaks me out, and even though I try I can not abide. It is neither better nor worse, is different.

So I would say, as I think, after a year of postdoc, that experience has been good, and to some extent talking idiomáticamemte enriching. It is true, you learn new techniques and others are repeated but with different adjustments also work (or not). But so far (just one day I despise), I see it shows that justifies that belief is so widespread that after foreign postdoctoral scientist is more valid than one that is stuck in your country. And still less, this type of postdoc is erected as essential premise in almost all offers or scholarships for postdocs who want to work in your country. Perhaps this was useful in a global world as the 20 -40 years ago, (not like that he lives in our generation) which leave the country meant to see the world completely hidden, and also access to the bases of development science and technology had not yet fully come to Spain. I think we had the good fortune to acquire this global knowledge site (for lack of ease with the language), and for us the rich experience was a postdoc at the time of our parents, it is much less.

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The first man in space

I think I can say without a doubt the most impressive thing was the change of the sky. The immediate step so pale in the morning to a black dotted with stars that appeared suddenly was something I will never forget. It is true that weightlessness is an incredible experience, but I had trained for it and had experienced at least partially, in-flight preparations, so artificial. But I was as surprised as the feeling of having left behind the planet. On the monitor screen still see the surface, not unlike one of those satellite photos are so common to everyone, but what I saw before me was not comparable to any photo, any representation that was very detailed. Anticipated So this time, he feared me down, perhaps readers have ever felt that DESPAGE, this long-anticipated moment when it finally occurs to be more bland and less special as we had imagined. Well, that was one of my biggest fears: imagine that the strong attachment to the seat, take-off nerves, the tension of the initial acceleration would leave me stunned and unable to enjoy the show, nothing is further from the truth. It was impossible to avoid such beauty.

take my eyes of the planet's surface that slowly slid to the left of the window, to find against the mighty moon. Someday I said, another would step fortunate that surface. Through the open door that had just one day we might consider the area of \u200b\u200bour home planet from different points of view, not only in a rudimentary orbit spacecraft as one in which I was, but from another rocky surface like the moon shining in front of me or your partner, on the other side of the planet. I wondered, in the middle of my ramblings, if my name would be as remembered as the first human to set foot on Phobos or Deimos. But the mysteries of history are that, mysteries, probably never get to know who was actually the man who gave his name to my ship and my mission. Mission I had become the first man launched into the orbit of Mars, after centuries since the first human had set foot in her red and barren land. Centuries of darkness and back, plus continuous years of revolutionary technological advances, hiding the tracks of the first cosmonauts, because since humans left the earth had lost much of the testimony of his days there. We will never know if it was the first to tread the moon of the earthlings to leave their planet, or simply look at the sky and distinguish the bodies orbiting around the same sun that now baffles me. Was only aware of the importance of his name, that at some point that name was associated with the space, man's liberation from bondage, to the realization of a dream.

have asked me to write these lines registered to leave my feelings after this important mission. For that to happen again forgetting the exploits that show what humans can. I am living testimony to a civilization is able to return to the place reached years ago: we have returned to space after centuries of condemnation to a planet that never was the origin of our species, much as we did ours. Perhaps because of these words some day my name will be remembered, and named one new spacecraft carrying the descendants of our species even further to the stars, like Yuri Gagarin, whoever he was, lent his name to the ship that now has allowed me to be part of history.

This entry is a modest tribute to Yuri Gagarin, the first man to travel into space on April 12, 1961, and is part of the initiative Yuriesfera .

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Patatins and other names of implausible, The Dairy

Dear friends, today I have the honor of presenting a contribution of our exile friend El Lechero , Which many know as a frequent commentator and one of the main drivers of the Darwinian idea insane. Humble as any good scientist, El Lechero has never wanted to officially sign the blog author and limited to working in the shadows, so sent me a fantastic text to publish it in your name (as it did on this one occasion with friends exiled in Germany).

Without further ado, I present, fresh from the USA, El Lechero!:

A lack of defined three-dimensional structure the protagonists of this article,

I took the liberty to illustrate the post with this metaphorical image.

Recently I had one of those days when you get excited by science and began to review literature, print articles and even to read, I understand ...

The fact is that in the midst of this fever, I found a protein called patatin "and I began to remember the funny names that are often proteins. Have been described many proteins and have been so complicated cellular processes that can not be known and that of a protein called "glycogen synthase. My mind began to move away from the article and think more and more of these proteins with funny name ... and I said "Darwinnnnnnnnnnnnnn !!!!!!!!!!! This I have to write in Jindetrés, go!".

Naturally, people are years and years researching, tracking unsuccessful so the day that leaves a positive and find a new protein, not waste this moment of glory for a baptism like and just go all out.

For example, what do you think of the oncogene Pokemon (POK erythroid myeloid ontogenic factor)? Other names have made the leap to the big screen as the scientific press R2D2, a protein involved in processing miRNAs, Groucho (not to be confused with our scholarship blog) ... and even Homer Simpson .

At one time also marked tendency that its protein baptized with names of animals: there are PUMA , Armadillo , Raptor Or Sonic Hedgehog. I personally find it very inspiring the name of the transcription factor " FOX." Each translate it as you like for me that meant "slut" and forgive the audacity. I also once met with the protein SIAH (seven in Absence ). Clearly, the day he was named in the laboratory had been smoking some firecrackers.

Other proteins have already been mentioned in this forum, and we are quite familiar but not want to be left out of this review. For example the family of proteins called SUMO or the E3 ubiquitin ligase called Malina .

A protein that life was known as PTG ( Protein targeting to glycogen ) the moment someone wanting to get dizzy, or messing R5 was renamed . Apart from the simple rhymes, I think he did it as a nostalgic tribute to the car in which many of us were conceived.

Continuing somewhat scatological names are " caspases," the " Culinas " or "Coitus interruptus , a protein described in D.melanogaster , and I'm not kidding. People who work in plants is not far behind. For example there AMC (Abstinence by mutual consent) protein described in Arabidopsis , or proteins Argonauts, of which already spoke here at the time .

Ultimately the joke and leave tiny footprints imagination in science. It seems that reality is sometimes stranger than fiction. In light of these data does not surprise me that the route composed of proteins such as lonchasoma , adobo1 and tomaquitilating proteins ( Dr. Litos, Banchsinger et al.; Jindetrés, salt!, 2010 ) charged a prophetic aspect to one day become a reality.

El Lechero

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Al sandwich without gown

is a pleasant morning of a brand new spring. Temperature, neither too cold nor too warm, the day is bright, not be blinded. A gentle breeze, which is received in the face with joy. The newly awakened city bustles with the comings and goings of people: the university area has its own fauna, especially around the hospital, where workers are mixed with patients, students and other pedestrians of all kinds.

During the customary googlélica documentation process for the post,

I found this interesting revelation: we have plagiarized Batablanca!

I cross a street, and go through the back of hospital facilities, on a sidewalk where a long succession of bars have been doing quite well thanks to the lunch time of local workers nearby. It would be hard to guess what is the origin of these workers, if it were not for ... a moment, in that group of people one of them is dressed all in green ... at the next table, I can distinguish a middle-aged man with a white coat over their clothes ... another table there, not one but two, not three people wearing hospital gowns, I'm crossing over between the tables in amazement ... Nurses with his green uniform! Wait, some of them look a little more, kind of jewelry around his neck ... Oh, no! It is a stethoscope , hanging gracefully, reflecting the rays of the sun as it swings hanging from the ravages doctoral students who take lunch.

contemplating this spectacle, a succession of images and ideas fills my troubled brain, imagine, suddenly, the stethoscope falling on the dish patatas bravas, and Doctor of ajoaceite surreptitiously wiping with a napkin (yes, those napkins bars, which are in uncomfortable boxes with sharp metal edges and are made less absorbent material on the face of the earth). Then imagine the same stethoscope on the chest of an innocent young woman, as she wonders why you are entering auscultated want to go for some beers. I imagine the nurse also shed his uniform on a plate lace, jumped up and facing the ashtray (remember, on the terraces of the bars are still smoking, at least for now) and pouring the contents on it singed uniform, resulting in a curious mixture of batter and ash never, never entirely abandon that pledge. I see that nurse returning to their jobs, support packages on your lap while you sterile opening, ash and debris espolsando nail on the limpid and sterile (no longer) the operating room floor.

But my views have also fearful symmetry: guess that doctor who takes a quiet coffee while reading the free newspaper (publishing official sponsor of any lunch during work hours that price), reads, as I say, quietly, forgetting that a few minutes ago bronchitic patient has coughed very close to the same beat that now lands on the table where, minutes later, he lay a couple to be happy cuddle between minced and chopped a potato salad seasoned with succulent Mycoplasmas.

first thing that cause these images is an absolute outrage, of course: who these people are believed to play well with the hygiene of the places public, especially in places devoted to leisure Gourmet? What show responsibility towards their workplace, that temple of cleanliness and sterility to be a place of healing and care as a hospital?

But I must calm down, I think. There must be an explanation. I've never worked in a hospital, so know the customs that should be in terms of clothing, and their rules ahead of going out with her. Maybe just me is basing on my personal experience, because in my work also takes her robe. And in my work, I am in daily contact with all kinds of organisms and substances harmful to health, hence the use a gown to avoid contact with them. Caution is twofold: if I leave the lab, I leave my coat behind me as it could lead traces of these substances and spot other places, or worse, other people. I can not imagine that in hospitals is very different reasoning. Which makes me pose other possible explanations: one, could be that the daily tedium to disrobe every now and then finished by making users desist, and pure laziness to go to lunch without regard robe. Second, somehow, in the hospital world the show at all times to coat is an unmistakable sign of rank and authority, and doctors look for demonstration that are neither nurses or technicians, or anything else: they are DOCTORS (no eye PhD; not confuse). Go out with her robe open, which proudly waving flag. Either option seems so absurd in itself, I actively seek alternatives: I can only think that the need for nurses and doctors to be covered by the gown is such that they have chosen to leave only fair play to lunch outside the hospital. Hygienically be logical, although rationally stupid.

It may criticize me - I reflect, almost coming to my workplace and must settle the question - I am having no in mind that gowns and uniforms so reviled could be perfectly clean, the second option mentioned above, if true, would imply a total and absolute hygiene (provided, of nuances, upon returning from lunch were automatically discarded garment before entering contact the hospital and its patients) without me knowing. And there, I realize right away, lies the crux of the matter: I can not know . Lords attacks, I do not know where has your gown or where will be. But that pledge has been invented to avoid contact with beings and substances potentially harmful, uncomfortable or simply disgusting to humans and therefore correlated with a sense of revulsion, disgust and unhealthy. It is, therefore, morally healthy wear it in an environment close to the intake of food. And this being very strict, and it also blows me apetecería meet people on the bus, cinema, or bowling.

So taking advantage of this open discussion forum is a web site, launched this question as absurd reflection with the hope that someone meets me once and for all the question of why it is so assumed that the vicinity of hospital facilities and public places to ride near people, smoke, eat and drink wearing a dress that is designed main function (if not only) to serve as a receptacle for scab. Arguably is a garment designed to wear on dirt, not society.

Until you get that response, I will very proudly reciting the motto I read in one of the laboratories of the faculty (of Medicine, precisely) in a nice poster illustrated by Ortifus for prevention campaign risk of Universitat de València :

(translated into Castilian would be "The sandwich, no gown")

A rhyme so simple that looking back I realize you do not need to read more than the title of the post to understand what that meant, since a blog is the worst enemy of the ability to synthesize .

What you eat lunch right.

And if not, prepare to suffer the righteous anger of Batam.

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swashbuckling Philosophy: Ethics in fiction

What they have in common the manga, comics, science fiction and fantasy literature? Reading or viewing most of the works belonging to these genera is considered by most as an entertainment for children's minds, or unfit for an adult but, apart from shooting and explosions, can this type of work to bring us any more edifying value?

Let's start with the example known to all Dragon Ball , that our country was so popularly received by the younger segments as relentlessly pursued by the parents' associations, and is that high doses of physical violence present in it (especially from the Z series) were considered clearly harmful to the sensitive minds of our children. I will not deny that most of us was precisely what attracted us to the series in our childhood fighting, fast action and explosions. But over the years, the image that remains in the mind is markedly different: remember Goku, the paragon of goodness and innocence, which showed us that with hard work and dedication always be overcome. Goku, always ready to defend others, to reach out to both the friend and the enemy, while maintaining confidence that even his worst rival could change and rehabilitation. Goku, who did not care that his best friend was green, with antennas on his forehead (as well as other anatomical characteristics quite shocking) because the important thing was inside.

One advantage of fiction is that you can communicate ideas in a more pure, because the real story is, in my opinion, two major drawbacks: it is never totally objective (as it normally is written unilaterally, and post-reading can not capture the essence and motives of those who participated in it), and the other problem is that only shows how we are, not as we can be. Fiction instead allows a simplification of the situations that help to better transmission of ideas (similar to the simplifications that we use in science when addressing a real problem that involves too many variables to handle it) in addition to being transmitted in a much more suitable vehicle to reach most of the public. As an example of this I would cite Star Wars: leaving aside the powers and lightsabers, the Jedi were a social group that spent years of his life to training and empowerment of its qualities, was guided by a strict code of ethics and righteousness and dedicated volunteers and non-profit organization to defend others and maintain peace, not bad, right? All this simply talking about a series of more or less basic values, if we look eg the work of some great science fiction as Isaac Asimov (pictured, right next to the pins. Taken from here) we'll respond sociological analysis of how a society not so different from ours to certain conflicts, such as the emergence of an alternative energy source , clean and popular but able to pose a huge threat to humanity ( The Gods Themselves ) or a study of the human population response to certain types of situations ( Foundation). Of course this does not mean that there are other highly moralistic readings,
widely accepted by society, but in my opinion Plato and Descartes are appropriate readings from the age of 28, if we want to educate our children and adolescents, it might have to seek an alternative approach that was able to convey a similar message in a way that they may be more acceptable to the senses.

Finally, I would cite one example: the wall-crawler our friend left us, and since its inception, with a maximum I think it would take to recover: "With great power comes great responsibility." Power is a word with multiple meanings, it is very easy to apply to individuals capable of lifting trucks like MicroMachines But what about us? Those born in the first world in the period of greatest human development to date, with the knowledge of our ancestors just a click away, the potential and possibilities are also a form of power, and since we granted the opportunity to develop as people, I think we have a moral obligation to use this power with knowledge, for the benefit of all. As we can see, the literature contains many examples, but where scientists can go looking for a reference? Who could learn that philosophy might be called "coat and pipette (in contrast to that of swashbuckling contained in the title).

figure becomes necessary to guide our scientists on the path of righteousness ... and this necessity is born a hero emblematic representative of the daily struggle in laboratories around the world with you ... BATAM!

minipreps Bash, avenging white collar

pipette fastest this side of western

(Figure Dr. Litos )

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planned obsolescence "? Reporters

Perhaps these are the last lines are written from our laboratory. Years of research come to an end, the most unexpected way: no budget cuts or pollution disasters, or corporate sabotage could end this once flourishing group of scientists, now reduced to a group of people without hope to continue their work . Begin the story from the beginning.

all started when the tabletop centrifuge failed first time. I had just started to turn the rotor, the screen showed a cryptic error message. Not remember who was the first that happened, but no one was alarmed too, because turning it off and then on solved the problem without more. However, soon the same happened to another colleague, and another, and so on, until you give a simple spin four tubes became a task that could last for several minutes. Unthinkable for the pace of work of a research laboratory in molecular biology. I remember one of the last times I bother to calculate that, throughout the purification process protein, had spent 12 hours just to centrifuge samples, which normally would have cost a total of approximately 1 hour, maximum. I also remember another time a friend, desperate, finished spinning their samples by pressing the rack against his belly while another classmate spinning wildly in a chair with wheels in the laboratory. At the time did not call too much attention, we even laugh a lot watching the poor return to the last resulting slurry by dizziness. For us they were simply things that happen in laboratories. The devices will occasionally do strange things.

But then the problem seemed to get it. A few days after the funny story of spinning chair, another of our colleagues opened the head when the agitator where incubábamos membranes and western blot gels Coomassiee rose from 110 rpm to 14500, without anyone touch. The lunchboxes were blasted in all directions, and anyone familiar with the kinetic energy and its devastating effects it will be understood that the blow dealt to the cause fainting companion immediately. Another of us had more luck, as only splashed the Coomassie other lunchbox. Was simply dyed blue from head to toe, which fortunately eased the tension, such as body image-avatareño pitufil aspect could not but provoke a general laugh. Well, except our friend who lay unconscious on the floor after the coup of the lunchbox. In any case, the anecdotes began to seem somewhat tiresome. And then everything crashed.

gradual failures in all units have accelerated exponentially. The sterility of crops booths left to function efficiently, and a host of undesirable microorganisms populated cell culture plates. We try to disinfect the entire crop room, so we went to thaw aliquots stored in liquid nitrogen: another futile effort, because the nitrogen tank had been leaking. It was scary to find a predoctoral researcher completely frozen. At first it was funny, it looked like the T1000 in the film, but we soon realized that the thing was getting serious, all cells had died, and indeed if he had no solution.

( source)

could not tell, if I wanted, one by one all the disasters that were happening. We decided to do an emergency meeting about our chief group, because seniority is a degree when there are problems of this nature in the laboratory. That was when we realized that for several days had gone. The last thing he knew was that it started out of town to participate in a dissertation, but apparently never reached its destination. We went down to talk to the security guard - who was busy trying to repair damaged security cameras (all) - and he said he had no record that our boss had left the building. Spare the reader the tedium of our investigations until we discovered that the lift stopped working with our head inside, we could understand him shouting for a while, but several hours ago and no answer. This was the straw that broke the camel: confused, like sheep without a shepherd to guide you, we've come to the lab, fearful of taking any initiative. Some have been better than others ... few minutes ago I went through the little room of trainees, where more young people have gathered around the coffee machine in the vain hope of it working again as if by magic. For several hours they were entertained with the facebook, until all the computers in the room stopped working. Under one of the tables, one of the youngest fellows clinging knees and rocks back and forth, whispering something about a scholarship that never charged for a computer failure.

( source)

do not know how to endure it. Many have left the building for days, but the rest of us are reaching the limits of our strength, as is evident from the last lines. Even has spread panic and superstition ... one of our technicians has jumped out the window, uttering cries about the end of the digital age and the apocalypse. Fortunately, with the broken window has cooled the atmosphere that was being stuffy air conditioning since collapsed.

Now that virtually

nothing we can do is when we start to think about what happened. Nothing in this laboratory that can be done only with our hands, without relying on machines. But more laps than we give the matter more strange looks. Some talk of sabotage ... that might explain the incident of the lift (the doctoral about to read his thesis would be the main suspect), but it seems unlikely that a laboratory of competition, working on the same topic, I had to take these steps to move forward in the publication of the result set. For this and have to referees. A possible government conspiracy is also ridiculous: the Ministry is simply cut funding as it has done in recent years. No, the reason should be beyond. Without resorting to superstition or pseudoscience (as I have told, was what led to our poor technical jump out the window), there is only one plausible explanation: obsolescence. A colleague recently saw a documentary speaking this concept, and apparently the theory that all devices have a limited life deliberately calculated accurately is something amply contrasted. But then to understand why all the devices have "expired" almost at the same time ... That is something that will require an arduous investigation. The question is, how can we find out, if we have no machines? "Without even an Internet connection? Because, although the computer from which I write is still responding, how much longer we ignore aguan

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Jindetrés, salt! (14): Heavy Metal from Poland

In this latest installment of Reporters Jindetrés, salt! , our former colleague and friend LeLizard presents us with a snapshot especially daunting. LeLizard is one of those cases of scientists with a split personality (like others in his spare time is dedicated to writing stories or paint monkeys, some even have blogs!): for the day is devoted to his doctoral thesis with passion and scientific rigor, while at night it becomes a popular DJ. In this portrait dedicated server can see it with your hands in the dough, and he who wants to dance to their tune and it is in Valencia, has only passed through the halls today deejing and Murrayclub , or day 20 in the Velvet .

On his trip to Poland, not only agreed to be photographed as a representative Jindetresiense: the guy has absolutely metalera currado a shirt and colossal. Read on to witness the metal.

The very legendary Deep Purple are making war.

Oh yeah!

never saw one more picture jevi.

LeLizard server knows that is absolute fan of Halloween ,
no doubt that this picture is a veiled tribute to one of the largest
metal bands of all time.

Yes, there was a picture even more jevi.

hope that this donation will inspire the thousands, nay thousands, millions of fans Jindetrés, salt! around the world. Our friend has set the bar quite high making up his own shirt and designing even a slogan, but remember that for friends participar en esta maravillosa iniciativa no se necesita más que papel, lápiz y una cámara, como ya demostraron en su momento las improvisadas pero no por ello menos emotivas instantáneas del compañero Banchsinger y nuestra fiel seguidora Juji .

Despido el post sugiriendo que tras terminar de leerlo, pinchéis cualquiera de los temas de esta lista , subáis el volumen hasta rozar la temeridad y entonéis en tono agudo y desgarrador el imponente lema:

¡Jindetrés, sal, y mucho metaaaaaal!